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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB1B Monoclonal antibody

RAB1B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C2G6

Cat no : CL488-67837

Print datasheet

Synonyms

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 3C2G6

验证数据展示

  • FC experiment of MCF-7 using CL488-67837

    FC experiment of MCF-7 using CL488-67837

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB1B (CL488-67837, Clone:3C2G6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-67837

    IF Staining of MCF-7 using CL488-67837

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 RAB1B antibody (CL488-67837, Clone: 3C2G6 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RAB1B Ready-To-Use IHC Kit. RAB1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67837-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67837 targets RAB1B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen RAB1B fusion protein Ag12069 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB1B, member RAS oncogene family
Synonyms Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 3C2G6
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC071169
Gene Symbol RAB1B
Gene ID (NCBI) 81876
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H0U4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB1B antibody CL488-67837Download protocol
FC protocol for CL Plus 488 RAB1B antibody CL488-67837Download protocol
Standard Protocols
Click here to view our Standard Protocols