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验证数据展示

  • FC experiment of NIH/3T3 using CL594-67837

    FC experiment of NIH/3T3 using CL594-67837

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAB1B (CL594-67837, Clone:3C2G6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of NIH/3T3 using CL594-67837

    IF Staining of NIH/3T3 using CL594-67837

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite®594 RAB1B antibody (CL594-67837, Clone: 3C2G6 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated RAB1B Monoclonal antibody

RAB1B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67837

产品说明书

CloneNo. 3C2G6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 3C2G6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy RAB1B Ready-To-Use IHC Kit. RAB1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67837-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67837 targets RAB1B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 RAB1B fusion protein Ag12069 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB1B, member RAS oncogene family
别名 Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 3C2G6
计算分子量 201 aa, 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC071169
基因名称 RAB1B
Gene ID (NCBI) 81876
RRIDAB_3084787
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H0U4
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 RAB1B antibody CL594-67837Download protocol
FC protocol for CL594 RAB1B antibody CL594-67837Download protocol
Standard Protocols
Click here to view our Standard Protocols