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RAB27B Monoclonal antibody

RAB27B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66944-1-Ig

产品说明书

CloneNo. 2B9G9

宿主/亚型

Mouse / IgG2a

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, human peripheral blood platelets, MCF-7 cells, HeLa cells, U-87 MG cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66944-1-Ig targets RAB27B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 RAB27B fusion protein Ag18756 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB27B, member RAS oncogene family
别名 C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9
计算分子量 218 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC027474
基因名称 RAB27B
Gene ID (NCBI) 5874
RRIDAB_2882268
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00194
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

实验方案

Product Specific Protocols
WB protocol for RAB27B antibody 66944-1-IgDownload protocol
IHC protocol for RAB27B antibody 66944-1-IgDownload protocol
IF protocol for RAB27B antibody 66944-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
humanWB,IF

J Biol Chem

The GTPase Rab27b regulates the release, autophagic clearance, and toxicity of α-synuclein.

Authors - Rachel Underwood
  • KD Validated
mouseIF

FEBS Open Bio

Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells.

Authors - Tomoko Kadowaki
humanWB

Am J Reprod Immunol

Rab27b promotes endometriosis by enhancing invasiveness of ESCs and promoting angiogenesis

Authors - Jing Shan
mouseIF

Front Cell Infect Microbiol

Uropathogenic Escherichia coli infection: innate immune disorder, bladder damage, and Tailin Fang II

Authors - Zong-Ping Li
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