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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RAB27B Monoclonal antibody

RAB27B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B9G9

Cat no : CL594-66944

Print datasheet

Synonyms

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9

验证数据展示

  • FC experiment of A431 using CL594-66944

    FC experiment of A431 using CL594-66944

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAB27B (CL594-66944, Clone:2B9G9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL594-66944

    IF Staining of A431 using CL594-66944

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 RAB27B antibody (CL594-66944, Clone: 2B9G9 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 66944-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66944 targets RAB27B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen RAB27B fusion protein Ag18756 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAB27B, member RAS oncogene family
Synonyms C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9
Calculated Molecular Weight 218 aa, 25 kDa
GenBank Accession NumberBC027474
Gene Symbol RAB27B
Gene ID (NCBI) 5874
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00194
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

实验方案

Product Specific Protocols
IF protocol for CL594 RAB27B antibody CL594-66944Download protocol
FC protocol for CL594 RAB27B antibody CL594-66944Download protocol
Standard Protocols
Click here to view our Standard Protocols