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验证数据展示

  • FC experiment of A431 using CL594-66944

    FC experiment of A431 using CL594-66944

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAB27B (CL594-66944, Clone:2B9G9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL594-66944

    IF Staining of A431 using CL594-66944

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 RAB27B antibody (CL594-66944, Clone: 2B9G9 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated RAB27B Monoclonal antibody

RAB27B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66944

产品说明书

CloneNo. 2B9G9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 66944-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66944 targets RAB27B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 RAB27B fusion protein Ag18756 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB27B, member RAS oncogene family
别名 C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9
计算分子量 218 aa, 25 kDa
GenBank蛋白编号BC027474
基因名称 RAB27B
Gene ID (NCBI) 5874
RRIDAB_2934751
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00194
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

实验方案

Product Specific Protocols
IF protocol for CL594 RAB27B antibody CL594-66944Download protocol
FC protocol for CL594 RAB27B antibody CL594-66944Download protocol
Standard Protocols
Click here to view our Standard Protocols