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验证数据展示

  • WB analysis using 84741-1-RR

    WB analysis using 84741-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84741-1-RR (RAB7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84741-1-RR

    IF Staining of A431 using 84741-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 84741-1-RR

    IF Staining of HeLa using 84741-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 84741-1-RR

    FC experiment of A431 using 84741-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of K-562 using 84741-1-RR

    FC experiment of K-562 using 84741-1-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

RAB7A Recombinant antibody

RAB7A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84741-1-RR

产品说明书

CloneNo. 241948B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

RAB7, Ras-related protein Rab-7a, Ras related protein Rab 7a, EC:3.6.5.2, 241948B4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, L-929 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells, HeLa cells
Positive FC (Intra) detected inK-562 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy RAB7A Ready-To-Use IHC Kit. RAB7A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84741-1-RR targets RAB7A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB7A, member RAS oncogene family
别名 RAB7, Ras-related protein Rab-7a, Ras related protein Rab 7a, EC:3.6.5.2, 241948B4
计算分子量 23 kDa
观测分子量 23 kDa
GenBank蛋白编号NM_004637
基因名称 RAB7A
Gene ID (NCBI) 7879
RRIDAB_3672126
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP51149
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

实验方案

Product Specific Protocols
WB protocol for RAB7A antibody 84741-1-RRDownload protocol
IF protocol for RAB7A antibody 84741-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols