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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84741-1-RR (same clone as 84741-1-PBS)

    WB analysis using 84741-1-RR (same clone as 84741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84741-1-RR (RAB7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84741-1-RR (same clone as 84741-1-PBS)

    IF Staining of A431 using 84741-1-RR (same clone as 84741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84741-1-RR (same clone as 84741-1-PBS)

    IF Staining of HeLa using 84741-1-RR (same clone as 84741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84741-1-RR (same clone as 84741-1-PBS)

    FC experiment of A431 using 84741-1-RR (same clone as 84741-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 84741-1-RR (same clone as 84741-1-PBS)

    FC experiment of K-562 using 84741-1-RR (same clone as 84741-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

RAB7A Recombinant antibody, PBS Only

RAB7A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84741-1-PBS

产品说明书

CloneNo. 241948B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

RAB7, Ras-related protein Rab-7a, Ras related protein Rab 7a, EC:3.6.5.2, 241948B4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAB7A Ready-To-Use IHC Kit. RAB7A primary antibody included.

产品信息

84741-1-PBS targets RAB7A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB7A, member RAS oncogene family
别名 RAB7, Ras-related protein Rab-7a, Ras related protein Rab 7a, EC:3.6.5.2, 241948B4
计算分子量 23 kDa
观测分子量 23 kDa
GenBank蛋白编号NM_004637
基因名称 RAB7A
Gene ID (NCBI) 7879
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP51149
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.