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RAC1 Monoclonal antibody

RAC1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66122-1-Ig

产品说明书

CloneNo. 4A4B11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, NIH/3T3 cells, HeLa cells, HUVEC cells, HL-60 cells, HSC-T6 cells, RAW 264.7 cells
Positive IP detected inHL-60 cells, HSC-T6 cells, Raw 264.7 cells, HUVEC cells, HL-60 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAC1 Ready-To-Use IHC Kit. RAC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66122-1-Ig targets RAC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, PLA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 RAC1 fusion protein Ag17779 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
别名 MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC004247
基因名称 RAC1
Gene ID (NCBI) 5879
RRIDAB_2881521
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP63000
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

实验方案

Product Specific Protocols
WB protocol for RAC1 antibody 66122-1-IgDownload protocol
IHC protocol for RAC1 antibody 66122-1-IgDownload protocol
IF protocol for RAC1 antibody 66122-1-IgDownload protocol
IP protocol for RAC1 antibody 66122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.

Authors - Huafeng Zhang
humanPLA

Nat Commun

Regulation of local GTP availability controls RAC1 activity and cell invasion

Authors - Anna Bianchi-Smiraglia
mouseWB

Nat Commun

TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection.

Authors - Jing Geng
mouseWB

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma
humanWB

Oncogene

Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Authors - Xin Jin
humanWB

Cell Chem Biol

Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.

Authors - David W Wolff
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