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RAC1 Monoclonal antibody
RAC1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat and More (1)
Applications
IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4A4B11
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, human brain tissue, NIH/3T3 cells, HeLa cells, HUVEC cells, HL-60 cells, HSC-T6 cells, RAW 264.7 cells |
Positive IP detected in | HL-60 cells, HSC-T6 cells, Raw 264.7 cells, HUVEC cells, HL-60 cells, NIH/3T3 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
66122-1-Ig targets RAC1 in WB, PLA, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | IF, IHC, IP, PLA, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | RAC1 fusion protein Ag17779 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
Synonyms | MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25 |
Calculated molecular weight | 21 kDa |
Observed molecular weight | 21 kDa |
GenBank accession number | BC004247 |
Gene symbol | RAC1 |
Uniprot | P63000 |
Gene ID (NCBI) | 5879 |
RRID | AB_2881521 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
实验方案
Product Specific Protocols | |
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WB protocol for RAC1 antibody 66122-1-Ig | Download protocol |
IHC protocol for RAC1 antibody 66122-1-Ig | Download protocol |
IF protocol for RAC1 antibody 66122-1-Ig | Download protocol |
IP protocol for RAC1 antibody 66122-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells. | ||
Nat Commun TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection. | ||
Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. | ||
Oncogene Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma. | ||
Cell Chem Biol Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4. |