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RAC1 Monoclonal antibody

RAC1 Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat and More (1)


IF/ICC, IHC, IP, WB, ELISA and More (2)





Cat No : 66122-1-Ig

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MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25


Positive WB detected inHEK-293 cells, human brain tissue, NIH/3T3 cells, HeLa cells, HUVEC cells, HL-60 cells, HSC-T6 cells, RAW 264.7 cells
Positive IP detected inHL-60 cells, HSC-T6 cells, Raw 264.7 cells, HUVEC cells, HL-60 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, HeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.


Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


66122-1-Ig targets RAC1 in WB, IP, IF, PLA, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IF, PLA, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen RAC1 fusion protein Ag17779 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Synonyms MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC004247
Gene Symbol RAC1
Gene ID (NCBI) 5879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.


Product Specific Protocols
WB protocol for RAC1 antibody 66122-1-IgDownload protocol
IHC protocol for RAC1 antibody 66122-1-IgDownload protocol
IF protocol for RAC1 antibody 66122-1-IgDownload protocol
IP protocol for RAC1 antibody 66122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.

Authors - Huafeng Zhang

Nat Commun

Regulation of local GTP availability controls RAC1 activity and cell invasion

Authors - Anna Bianchi-Smiraglia

Nat Commun

TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection.

Authors - Jing Geng

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma


Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Authors - Xin Jin

Cell Chem Biol

Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.

Authors - David W Wolff