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CoraLite® Plus 488-conjugated RAC1 Monoclonal antibody
RAC1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
IF/ICC and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4A4B11
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66122-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
The immunogen of CL488-66122 is RAC1 Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | RAC1 fusion protein Ag17779 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
Synonyms | MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25 |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC004247 |
Gene Symbol | RAC1 |
Gene ID (NCBI) | 5879 |
RRID | AB_2883264 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P63000 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 RAC1 antibody CL488-66122 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Death Dis MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling. |