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验证数据展示

  • WB analysis using 66122-1-Ig (same clone as 66122-1-PBS)

    WB analysis using 66122-1-Ig (same clone as 66122-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66122-1-Ig (same clone as 66122-1-PBS)

    WB analysis of NIH/3T3 using 66122-1-Ig (same clone as 66122-1-PBS)

    WB result of RAC1 antibody (66122-1-Ig, 1:2000) with si-control and si-RAC1 transfected NIH3T3 cells. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66122-1-Ig (same clone as 66122-1-PBS)

    IHC staining of human colon cancer using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAC1 antibody included
  • IHC staining of human colon cancer using 66122-1-Ig (same clone as 66122-1-PBS)

    IHC staining of human colon cancer using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAC1 antibody included
  • IF Staining of NIH/3T3 using 66122-1-Ig (same clone as 66122-1-PBS)

    IF Staining of NIH/3T3 using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • IP experiment of HL-60 using 66122-1-Ig (same clone as 66122-1-PBS)

    IP experiment of HL-60 using 66122-1-Ig (same clone as 66122-1-PBS)

    IP result of anti-RAC1 (IP:66122-1-Ig, 3ug; Detection:66122-1-Ig 1:700) with HL-60 cells lysate 4000ug. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RAC1 Monoclonal antibody, PBS Only

RAC1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66122-1-PBS

产品说明书

CloneNo. 4A4B11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RAC1 Ready-To-Use IHC Kit. RAC1 primary antibody included.

产品信息

66122-1-PBS targets RAC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17779

Product name: Recombinant human RAC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-192 aa of BC004247

Sequence: MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
别名 MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC004247
基因名称 RAC1
Gene ID (NCBI) 5879
RRIDAB_2881521
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP63000
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.