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验证数据展示

  • IF Staining of HeLa using CL488-66940

    IF Staining of HeLa using CL488-66940

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 RACK1; GNB2L1 antibody (CL488-66940, Clone: 1E11A7 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated RACK1; GNB2L1 Monoclonal antibody

RACK1; GNB2L1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66940

产品说明书

CloneNo. 1E11A7

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF/ICC

别名

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RACK1 Ready-To-Use IHC Kit. RACK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66940-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66940 targets RACK1; GNB2L1 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 RACK1; GNB2L1 fusion protein Ag26708 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
别名 Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1
计算分子量 36 kDa
GenBank蛋白编号BC019093
基因名称 RACK1
Gene ID (NCBI) 10399
RRIDAB_2919393
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP63244
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RACK1; GNB2L1 antibody CL488-66940Download protocol
Standard Protocols
Click here to view our Standard Protocols