验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-66940 targets RACK1; GNB2L1 in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human |
| 文献引用反应性 | mouse |
| 免疫原 | RACK1; GNB2L1 fusion protein Ag26708 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 |
| 别名 | Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1 |
| 计算分子量 | 36 kDa |
| GenBank蛋白编号 | BC019093 |
| 基因名称 | RACK1 |
| Gene ID (NCBI) | 10399 |
| RRID | AB_2920060 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P63244 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 RACK1; GNB2L1 antibody CL594-66940 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |