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  • Featured Product
  • KD/KO Validated

RBAP48 Monoclonal antibody, PBS Only

RBAP48 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C4D6

Cat no : 66060-1-PBS

Print datasheet

Synonyms

RBBP4, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48, CAF-I 48 kDa subunit

验证数据展示

  • WB analysis using 66060-1-Ig (same clone as 66060-1-PBS)

    WB analysis using 66060-1-Ig (same clone as 66060-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66060-1-Ig (RBAP48 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66060-1-Ig (same clone as 66060-1-PBS)

    IF Staining of HepG2 using 66060-1-Ig (same clone as 66060-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66060-1-Ig (RBAP48 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66060-1-Ig (same clone as 66060-1-PBS)

    IHC staining of human cervical cancer using 66060-1-Ig (same clone as 66060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBBP4 antibody included
  • IHC staining of human cervical cancer using 66060-1-Ig (same clone as 66060-1-PBS)

    IHC staining of human cervical cancer using 66060-1-Ig (same clone as 66060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBBP4 antibody included
  • FC experiment of HepG2 using 66060-1-Ig (same clone as 66060-1-PBS)

    FC experiment of HepG2 using 66060-1-Ig (same clone as 66060-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human RBAP48 (66060-1-Ig, Clone:5C4D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66060-1-Ig (same clone as 66060-1-PBS)

    WB analysis of HeLa using 66060-1-Ig (same clone as 66060-1-PBS)

    WB result of RBAP48 antibody (66060-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells. This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RBBP4 Ready-To-Use IHC Kit. RBBP4 primary antibody included.

产品信息

66060-1-PBS targets RBAP48 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen RBAP48 fusion protein Ag6196 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name retinoblastoma binding protein 4
Synonyms RBBP4, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48, CAF-I 48 kDa subunit
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC053904
Gene Symbol RBBP4
Gene ID (NCBI) 5928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09028
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).