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RBAP48 Monoclonal antibody

RBAP48 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5C4D6

Cat no : 66060-1-Ig

Print datasheet

Synonyms

RBBP4, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48, CAF-I 48 kDa subunit

验证数据展示

  • WB analysis using 66060-1-Ig

    WB analysis using 66060-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66060-1-Ig (RBAP48 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66060-1-Ig

    IF Staining of HepG2 using 66060-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66060-1-Ig (RBAP48 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human cervical cancer using 66060-1-Ig

    IHC staining of human cervical cancer using 66060-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RBBP4 antibody included
  • IHC staining of human cervical cancer using 66060-1-Ig

    IHC staining of human cervical cancer using 66060-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with RBBP4 antibody included
  • FC experiment of HepG2 using 66060-1-Ig

    FC experiment of HepG2 using 66060-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human RBAP48 (66060-1-Ig, Clone:5C4D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis of HeLa using 66060-1-Ig

    WB analysis of HeLa using 66060-1-Ig

    WB result of RBAP48 antibody (66060-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, RAW 264.7 cells, Jurkat cell, ROS1728 cells, HSC-T6 cells, L02 cells
Positive IHC detected inhuman cervical cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RBBP4 Ready-To-Use IHC Kit. RBBP4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66060-1-Ig targets RBAP48 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RBAP48 fusion protein Ag6196 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name retinoblastoma binding protein 4
Synonyms RBBP4, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48, CAF-I 48 kDa subunit
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC053904
Gene Symbol RBBP4
Gene ID (NCBI) 5928
RRIDAB_11064731
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09028
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

实验方案

Product Specific Protocols
WB protocol for RBAP48 antibody 66060-1-IgDownload protocol
IHC protocol for RBAP48 antibody 66060-1-IgDownload protocol
IF protocol for RBAP48 antibody 66060-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai
View Article