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  • Featured Product
  • KD/KO Validated

RBM15 Monoclonal antibody

RBM15 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4A1A4

Cat no : 66059-1-Ig

Print datasheet

Synonyms

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

验证数据展示

  • WB analysis using 66059-1-Ig

    WB analysis using 66059-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human breast cancer using 66059-1-Ig

    IHC staining of human breast cancer using 66059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM15 antibody included
  • IF Staining of HeLa using 66059-1-Ig

    IF Staining of HeLa using 66059-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (66059-1-Ig, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IP experiment of HEK-293 using 66059-1-Ig

    IP experiment of HEK-293 using 66059-1-Ig

    IP result of anti-RBM15 (IP:66059-1-Ig, 3ug; Detection:66059-1-Ig 1:500) with HEK-293 cells lysate 1800ug.

  • WB analysis of HeLa using 66059-1-Ig

    WB analysis of HeLa using 66059-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK293 cells using 66059-1-Ig

    WB analysis of HEK293 cells using 66059-1-Ig

    WB result of RBM15 antibody (66059-1-Ig, 1:4000) with si-control and si-RBM15 transfected HEK293 cells.

经过测试的应用

Positive WB detected inA549 cells, human brain tissue, HeLa cells, human heart tissue, HEK-293 cells, HEK293 cells, MCF-7 cells, LNCaP cells, Jurkat cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RBM15 Ready-To-Use IHC Kit. RBM15 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66059-1-Ig targets RBM15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen RBM15 fusion protein Ag17970 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RNA binding motif protein 15
Synonyms One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC006397
Gene Symbol RBM15
Gene ID (NCBI) 64783
RRIDAB_11042326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96T37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

实验方案

Product Specific Protocols
WB protocol for RBM15 antibody 66059-1-IgDownload protocol
IHC protocol for RBM15 antibody 66059-1-IgDownload protocol
IF protocol for RBM15 antibody 66059-1-IgDownload protocol
IP protocol for RBM15 antibody 66059-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Nat Commun

Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.

Authors - Yuxian Guo
View Article
humanWB

Elife

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

Authors - Li Zhang
View Article
WB

EMBO Rep

The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation.

Authors - Ngoc-Tung Tran
View Article
humanIHC

J Gene Med

The pathological tissue expression pattern and clinical significance of m6A-regulatory genes in non-small cell lung cancer.

Authors - Yujie Li
View Article