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验证数据展示

  • IF Staining of HeLa using CL594-66059

    IF Staining of HeLa using CL594-66059

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 RBM15 antibody (CL594-66059, Clone: 4A1A4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66059

    FC experiment of HeLa using CL594-66059

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM15 (CL594-66059, Clone:4A1A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RBM15 Monoclonal antibody

RBM15 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66059
CloneNo. 4A1A4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

RNA-binding motif protein 15, RBM 15, OTT1, OTT, One-twenty two protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RBM15 Ready-To-Use IHC Kit. RBM15 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66059-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66059 targets RBM15 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen RBM15 fusion protein Ag17970 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RNA binding motif protein 15
Synonyms RNA-binding motif protein 15, RBM 15, OTT1, OTT, One-twenty two protein 1
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC006397
Gene Symbol RBM15
Gene ID (NCBI) 64783
RRIDAB_2919943
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96T37
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

实验方案

Product Specific Protocols
IF protocol for CL594 RBM15 antibody CL594-66059Download protocol
FC protocol for CL594 RBM15 antibody CL594-66059Download protocol
Standard Protocols
Click here to view our Standard Protocols