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CoraLite® Plus 488-conjugated RBM16 Polyclonal antibody

RBM16 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18893

Print datasheet

Synonyms

RBM16, RNA binding protein 16, RNA-binding motif protein 16, SCAF8, SR-related and CTD-associated factor 8, CCAP7, CDC5L complex-associated protein 7, KIAA1116

验证数据展示

  • IF Staining of HeLa using CL488-18893

    IF Staining of HeLa using CL488-18893

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RBM16 antibody (CL488-18893) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-18893

    FC experiment of HeLa using CL488-18893

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated RBM16 Polyclonal antibody (CL488-18893)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SCAF8 Ready-To-Use IHC Kit. SCAF8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18893-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18893 targets RBM16 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen RBM16 fusion protein Ag13499 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 16
Synonyms RBM16, RNA binding protein 16, RNA-binding motif protein 16, SCAF8, SR-related and CTD-associated factor 8, CCAP7, CDC5L complex-associated protein 7, KIAA1116
Calculated Molecular Weight 1271 aa, 141 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC070071
Gene Symbol RBM16
Gene ID (NCBI) 22828
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPN6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RBM16 antibody CL488-18893Download protocol
FC protocol for CL Plus 488 RBM16 antibody CL488-18893Download protocol
Standard Protocols
Click here to view our Standard Protocols