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验证数据展示

  • IF Staining of HepG2 using 82889-1-RR (same clone as 82889-1-PBS)

    IF Staining of HepG2 using 82889-1-RR (same clone as 82889-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM17 antibody (82889-1-RR, Clone: 230125F6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82889-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 82889-1-RR (same clone as 82889-1-PBS)

    WB analysis of MCF-7 using 82889-1-RR (same clone as 82889-1-PBS)

    WB result of RBM17 antibody (82889-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells. This data was developed using the same antibody clone with 82889-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 82889-1-RR (same clone as 82889-1-PBS)

    IP experiment of MCF-7 using 82889-1-RR (same clone as 82889-1-PBS)

    IP result of anti-RBM17 (IP:82889-1-RR, 4ug; Detection:82889-1-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82889-1-RR (same clone as 82889-1-PBS)

    FC experiment of HepG2 using 82889-1-RR (same clone as 82889-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBM17 Recombinant antibody (82889-1-RR, Clone:230125F6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82889-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82889-1-RR

    Affinity and Kinetic Characterization of 82889-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-1-RR against Human RBM17 were performed. The affinity constant is 1.54 nM.

  • Featured Product
  • KD/KO验证

RBM17 Recombinant antibody, PBS Only

RBM17 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82889-1-PBS

产品说明书

CloneNo. 230125F6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82889-1-PBS targets RBM17 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 RBM17 fusion protein Ag0985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 17
别名 SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor
计算分子量 45 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC007871
基因名称 RBM17
Gene ID (NCBI) 84991
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96I25
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.