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验证数据展示

  • IF Staining of HepG2 using 82889-1-RR

    IF Staining of HepG2 using 82889-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM17 antibody (82889-1-RR, Clone: 230125F6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

  • WB analysis of MCF-7 using 82889-1-RR

    WB analysis of MCF-7 using 82889-1-RR

    WB result of RBM17 antibody (82889-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells.

  • IP experiment of MCF-7 using 82889-1-RR

    IP experiment of MCF-7 using 82889-1-RR

    IP result of anti-RBM17 (IP:82889-1-RR, 4ug; Detection:82889-1-RR 1:10000) with MCF-7 cells lysate 1470 ug.

  • FC experiment of HepG2 using 82889-1-RR

    FC experiment of HepG2 using 82889-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBM17 Recombinant antibody (82889-1-RR, Clone:230125F6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82889-1-RR

    Affinity and Kinetic Characterization of 82889-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-1-RR against Human RBM17 were performed. The affinity constant is 1.54 nM.

  • Featured Product
  • KD/KO验证

RBM17 Recombinant antibody

RBM17 Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 82889-1-RR

产品说明书

CloneNo. 230125F6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IP detected inMCF-7 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82889-1-RR targets RBM17 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 RBM17 fusion protein Ag0985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 17
别名 SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor
计算分子量 45 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC007871
基因名称 RBM17
Gene ID (NCBI) 84991
RRIDAB_3670611
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96I25
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

实验方案

Product Specific Protocols
WB protocol for RBM17 antibody 82889-1-RRDownload protocol
IF protocol for RBM17 antibody 82889-1-RRDownload protocol
IP protocol for RBM17 antibody 82889-1-RRDownload protocol
FC protocol for RBM17 antibody 82889-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols