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CoraLite® Plus 488-conjugated RBM17 Recombinant antibody

RBM17 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230125F6

Cat no : CL488-82889

Print datasheet

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

验证数据展示

  • IF Staining of HepG2 using CL488-82889

    IF Staining of HepG2 using CL488-82889

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RBM17 antibody (CL488-82889, Clone: 230125F6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-82889

    FC experiment of HepG2 using CL488-82889

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated RBM17 Recombinant antibody (CL488-82889, Clone:230125F6)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82889-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82889 targets RBM17 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen RBM17 fusion protein Ag0985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RNA binding motif protein 17
Synonyms SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC007871
Gene Symbol RBM17
Gene ID (NCBI) 84991
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96I25
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RBM17 antibody CL488-82889Download protocol
FC protocol for CL Plus 488 RBM17 antibody CL488-82889Download protocol
Standard Protocols
Click here to view our Standard Protocols