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验证数据展示

  • IF Staining of HepG2 using CL488-82889

    IF Staining of HepG2 using CL488-82889

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RBM17 antibody (CL488-82889, Clone: 230125F6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-82889

    FC experiment of HepG2 using CL488-82889

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated RBM17 Recombinant antibody (CL488-82889, Clone:230125F6)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated RBM17 Recombinant antibody

RBM17 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82889

产品说明书

CloneNo. 230125F6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82889-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82889 targets RBM17 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 RBM17 fusion protein Ag0985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 17
别名 SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor
计算分子量 45 kDa
GenBank蛋白编号BC007871
基因名称 RBM17
Gene ID (NCBI) 84991
RRIDAB_3673099
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96I25
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RBM17 antibody CL488-82889Download protocol
FC protocol for CL Plus 488 RBM17 antibody CL488-82889Download protocol
Standard Protocols
Click here to view our Standard Protocols