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验证数据展示

  • WB analysis using 67335-1-Ig

    WB analysis using 67335-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67335-1-Ig (RCC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67335-1-Ig

    IHC staining of mouse brain using 67335-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RCC1 antibody included

RCC1 Monoclonal antibody

RCC1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67335-1-Ig

产品说明书

CloneNo. 2C2F5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RCC1 Ready-To-Use IHC Kit. RCC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67335-1-Ig targets RCC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 RCC1 fusion protein Ag17809 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 regulator of chromosome condensation 1
别名 Cell cycle regulatory protein, CHC1, RCC1, RCC1 I
计算分子量 421 aa, 45 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC010067
基因名称 RCC1
Gene ID (NCBI) 1104
RRIDAB_2882593
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP18754
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.

实验方案

Product Specific Protocols
WB protocol for RCC1 antibody 67335-1-IgDownload protocol
IHC protocol for RCC1 antibody 67335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols