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RCC1 Monoclonal antibody, PBS Only

RCC1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C2F5

Cat No : 67335-1-PBS

Print datasheet

Synonyms

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

验证数据展示

  • WB analysis using 67335-1-Ig (same clone as 67335-1-PBS)

    WB analysis using 67335-1-Ig (same clone as 67335-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67335-1-Ig (RCC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

    IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCC1 antibody included
Planning an IHC experiment? We recommend our IHCeasy RCC1 Ready-To-Use IHC Kit. RCC1 primary antibody included.

产品信息

67335-1-PBS targets RCC1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen RCC1 fusion protein Ag17809 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name regulator of chromosome condensation 1
Synonyms Cell cycle regulatory protein, CHC1, RCC1, RCC1 I
Calculated Molecular Weight 421 aa, 45 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC010067
Gene Symbol RCC1
Gene ID (NCBI) 1104
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18754
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.