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验证数据展示

  • WB analysis using 67335-1-Ig (same clone as 67335-1-PBS)

    WB analysis using 67335-1-Ig (same clone as 67335-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67335-1-Ig (RCC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

    IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCC1 antibody included

RCC1 Monoclonal antibody, PBS Only

RCC1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67335-1-PBS

产品说明书

CloneNo. 2C2F5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IHC, Indirect ELISA

别名

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RCC1 Ready-To-Use IHC Kit. RCC1 primary antibody included.

产品信息

67335-1-PBS targets RCC1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 RCC1 fusion protein Ag17809 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 regulator of chromosome condensation 1
别名 Cell cycle regulatory protein, CHC1, RCC1, RCC1 I
计算分子量 421 aa, 45 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC010067
基因名称 RCC1
Gene ID (NCBI) 1104
RRIDAB_2882593
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP18754
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.