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验证数据展示

  • IF Staining of HepG2 using CL594-67335

    IF Staining of HepG2 using CL594-67335

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RCC1 antibody (CL594-67335, Clone: 2C2F5 ) at dilution of 1:200. DAPI (blue).

CoraLite®594-conjugated RCC1 Monoclonal antibody

RCC1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67335
CloneNo. 2C2F5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RCC1 Ready-To-Use IHC Kit. RCC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67335-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67335 targets RCC1 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen RCC1 fusion protein Ag17809 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name regulator of chromosome condensation 1
Synonyms Cell cycle regulatory protein, CHC1, RCC1, RCC1 I
Calculated Molecular Weight 421 aa, 45 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC010067
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_2920115
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18754
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.

实验方案

Product Specific Protocols
IF protocol for CL594 RCC1 antibody CL594-67335Download protocol
Standard Protocols
Click here to view our Standard Protocols