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验证数据展示

  • WB analysis using 84892-1-RR

    WB analysis using 84892-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84892-1-RR (RFC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 84892-1-RR

    IHC staining of mouse ovary using 84892-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84892-1-RR (RFC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84892-1-RR

    IF Staining of HeLa using 84892-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC2 antibody (84892-1-RR, Clone: 242503E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84892-1-RR

    FC experiment of HeLa using 84892-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RFC2 Recombinant antibody (84892-1-RR, Clone:242503E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84892-1-RR

    Affinity and Kinetic Characterization of 84892-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84892-1-RR against Human RFC2 were performed. The affinity constant is 0.775 nM.

RFC2 Recombinant antibody

RFC2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84892-1-RR

Print datasheet

CloneNo. 242503E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, MCF-7 cells, Raji cells, NIH-3T3 cells, HSC-T6 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84892-1-RR targets RFC2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RFC2 fusion protein Ag0663 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name replication factor C (activator 1) 2, 40kDa
Synonyms Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, A1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC002813
Gene Symbol RFC2
Gene ID (NCBI) 5982
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35250
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFC2, also named RFC40, belongs to the activator 1 small subunits family. It is a 39 kDa Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).

实验方案

Product Specific Protocols
WB protocol for RFC2 antibody 84892-1-RRDownload protocol
IHC protocol for RFC2 antibody 84892-1-RRDownload protocol
IF protocol for RFC2 antibody 84892-1-RRDownload protocol
FC protocol for RFC2 antibody 84892-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols