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RFC2 Recombinant antibody, PBS Only (Capture)

RFC2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242503E12

Cat no : 84892-1-PBS

Print datasheet

Synonyms

Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, A1

验证数据展示

  • WB analysis using 84892-1-RR (same clone as 84892-1-PBS)

    WB analysis using 84892-1-RR (same clone as 84892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84892-1-RR (RFC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 84892-1-RR (same clone as 84892-1-PBS)

    IHC staining of mouse ovary using 84892-1-RR (same clone as 84892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84892-1-RR (RFC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    IF Staining of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC2 antibody (84892-1-RR, Clone: 242503E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    FC experiment of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RFC2 Recombinant antibody (84892-1-RR, Clone:242503E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01625-2

    Sandwich ELISA standard curve of MP01625-2

    Sandwich ELISA standard curve of MP01625-2, Human RFC2 Recombinant Matched Antibody Pair - PBS only. 84892-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0663. 84892-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01625-2

    Sample test of MP01625-2

    The mean RFC2 concentration was determined to be 55.11 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load, 130.72 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load and 43.49 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • Cytometric bead array standard curve of MP01625-2

    Cytometric bead array standard curve of MP01625-2

    Cytometric bead array standard curve of MP01625-2, RFC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84892-1-PBS. Detection antibody: 84892-2-PBS. Standard: Ag0663. Range: 0.625-80 ng/mL.

产品信息

84892-1-PBS targets RFC2 as part of a matched antibody pair:

MP01625-2: 84892-1-PBS capture and 84892-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen RFC2 fusion protein Ag0663 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name replication factor C (activator 1) 2, 40kDa
Synonyms Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, A1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC002813
Gene Symbol RFC2
Gene ID (NCBI) 5982
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35250
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RFC2, also named RFC40, belongs to the activator 1 small subunits family. It is a 39 kDa Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).