验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, C6 cells, Raji cells, SH-SY5Y cells, U-251 cells, Y79 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
66441-1-Ig targets RGS4 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, rat, mouse, pig |
| Cited Reactivity | mouse |
| Immunogen | RGS4 fusion protein Ag6029 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | regulator of G-protein signaling 4 |
| Synonyms | RGP4, RGS4, SCZD9 |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC051869 |
| Gene Symbol | RGS4 |
| Gene ID (NCBI) | 5999 |
| RRID | AB_2881811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49798 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RGS4 antibody 66441-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hippocampus NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus. | ||
J Cell Physiol MEOX1 triggers myofibroblast apoptosis resistance, contributing to pulmonary fibrosis in mice |
