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验证数据展示

  • WB analysis using CL488-66441

    WB analysis using CL488-66441

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66441 (RGS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL488-66441

    WB analysis using CL488-66441

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66441 (RGS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated RGS4 Monoclonal antibody

RGS4 Monoclonal Antibody for WB
Cat No. CL488-66441

产品说明书

CloneNo. 1E2E2

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, mouse, pig

应用

WB

别名

DKFZp761F1924, RGP4, RGS4, SCZD9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells, Neuro-2a cells, Raji cells, SH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 66441-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66441 targets RGS4 in WB applications and shows reactivity with human, rat, mouse, pig samples.

经测试应用 WB Application Description
经测试反应性 human, rat, mouse, pig
免疫原 RGS4 fusion protein Ag6029 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 regulator of G-protein signaling 4
别名 DKFZp761F1924, RGP4, RGS4, SCZD9
计算分子量 23 kDa
观测分子量 34 kDa
GenBank蛋白编号BC051869
基因名称 RGS4
Gene ID (NCBI) 5999
RRIDAB_3084246
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49798
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 RGS4 antibody CL488-66441Download protocol
Standard Protocols
Click here to view our Standard Protocols