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验证数据展示

  • WB analysis using 67542-1-Ig (same clone as 67542-1-PBS)

    WB analysis using 67542-1-Ig (same clone as 67542-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 67542-1-Ig (same clone as 67542-1-PBS)

    IHC staining of human small intestine using 67542-1-Ig (same clone as 67542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67542-1-Ig (RHOC antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67542-1-Ig (same clone as 67542-1-PBS)

    IF Staining of HeLa using 67542-1-Ig (same clone as 67542-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RHOC antibody (67542-1-Ig, Clone: 1G1C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 67542-1-Ig (same clone as 67542-1-PBS)

    FC experiment of Jurkat using 67542-1-Ig (same clone as 67542-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug RHOC Monoclonal antibody (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67542-1-Ig (same clone as 67542-1-PBS)

    FC experiment of HepG2 using 67542-1-Ig (same clone as 67542-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug RHOC Monoclonal antibody (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

RHOC Monoclonal antibody, PBS Only

RHOC Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67542-1-PBS

产品说明书

CloneNo. 1G1C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

ARHC, ARH9, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, H9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67542-1-PBS targets RHOC in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RHOC fusion protein Ag14633 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ras homolog gene family, member C
别名 ARHC, ARH9, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, H9
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC007245
基因名称 RHOC
Gene ID (NCBI) 389
RRIDAB_2882761
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08134
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.