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验证数据展示

  • WB analysis using 67542-1-Ig

    WB analysis using 67542-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 67542-1-Ig

    IHC staining of human small intestine using 67542-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67542-1-Ig (RHOC antibody) at dilution of 1:800 (under 40x lens).

  • IF Staining of HeLa using 67542-1-Ig

    IF Staining of HeLa using 67542-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RHOC antibody (67542-1-Ig, Clone: 1G1C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of Jurkat using 67542-1-Ig

    FC experiment of Jurkat using 67542-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug RHOC Monoclonal antibody (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using 67542-1-Ig

    FC experiment of HepG2 using 67542-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug RHOC Monoclonal antibody (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

RHOC Monoclonal antibody

RHOC Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67542-1-Ig

产品说明书

CloneNo. 1G1C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

ARHC, ARH9, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, H9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, C6 cells, NIH/3T3 cells, HEK-293 cells, PC-12 cells, RAW 264.7 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67542-1-Ig targets RHOC in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RHOC fusion protein Ag14633 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ras homolog gene family, member C
别名 ARHC, ARH9, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, H9
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC007245
基因名称 RHOC
Gene ID (NCBI) 389
RRIDAB_2882761
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08134
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.

实验方案

Product Specific Protocols
WB protocol for RHOC antibody 67542-1-IgDownload protocol
IHC protocol for RHOC antibody 67542-1-IgDownload protocol
IF protocol for RHOC antibody 67542-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols