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验证数据展示

  • IF Staining of HeLa using CL488-67542

    IF Staining of HeLa using CL488-67542

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RHOC antibody (CL488-67542, Clone: 1G1C1 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated RHOC Monoclonal antibody

RHOC Monoclonal Antibody for IF/ICC
Cat No. CL488-67542

Print datasheet

CloneNo. 1G1C1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

ARH9, ARHC, H9, Rho cDNA clone 9, RHOC, RHOH9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67542-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67542 targets RHOC in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen RHOC fusion protein Ag14633 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ras homolog gene family, member C
Synonyms ARH9, ARHC, H9, Rho cDNA clone 9, RHOC, RHOH9
Calculated Molecular Weight 22 kDa
GenBank Accession NumberBC007245
Gene Symbol RHOC
Gene ID (NCBI) 389
RRIDAB_2919497
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08134
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RHOC antibody CL488-67542Download protocol
Standard Protocols
Click here to view our Standard Protocols