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CoraLite® Plus 488-conjugated RIAM,APBB1IP Monoclonal antibody

RIAM,APBB1IP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F8A2

Cat no : CL488-67143

Print datasheet

Synonyms

APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP

验证数据展示

  • IF Staining of Jurkat using CL488-67143

    IF Staining of Jurkat using CL488-67143

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 RIAM,APBB1IP antibody (CL488-67143, Clone: 2F8A2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 67143-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67143 targets RIAM,APBB1IP in IF/ICC applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse
Immunogen RIAM,APBB1IP fusion protein Ag4602 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
Synonyms APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP
Calculated Molecular Weight 666 aa, 73 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC035636
Gene Symbol RIAM
Gene ID (NCBI) 54518
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z5R6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RIAM,APBB1IP antibody CL488-67143Download protocol
Standard Protocols
Click here to view our Standard Protocols