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CoraLite® Plus 488-conjugated RIF1 Polyclonal antibody

RIF1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-30119

Print datasheet

Synonyms

DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1

验证数据展示

  • IF Staining of HEK-293 using CL488-30119

    IF Staining of HEK-293 using CL488-30119

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 RIF1 antibody (CL488-30119) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RIF1 Ready-To-Use IHC Kit. RIF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 30119-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30119 targets RIF1 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen RIF1 fusion protein Ag32422 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAP1 interacting factor homolog (yeast)
Synonyms DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1
Calculated Molecular Weight274 kDa
Observed Molecular Weight300 kDa
GenBank Accession NumberNM_001177663.1
Gene Symbol RIF1
Gene ID (NCBI) 55183
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5UIP0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIF1 (Rap1-interacting factor 1) is a multifunctional chromosome stability protein implicated in controlling DNA replication and repair. Human RIF1 can interact with protein phosphatase 1 (PP1) and the interaction is crucial for controlling DNA replication by limiting phosphorylation of the MCM complex (PMID: 28077461). RIF1 also acts with PP1 to protect nascent DNA from overdegradation at stalled replication forks (PMID: 31141682). RIF1 promotes non-homologous end joining (NHEJ) in double strand break (DSB) repair (PMID: 30443189). Research studies show that RIF1 is overexpressed in breast cancer tissues and is upgraded in NSCLC tissues (PMID: 30237512).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RIF1 antibody CL488-30119Download protocol
Standard Protocols
Click here to view our Standard Protocols