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CoraLite® Plus 488-conjugated RIG-1/DDX58 Monoclonal antibody

RIG-1/DDX58 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B10F5

Cat no : CL488-67556

Print datasheet

Synonyms

DDX58, DEAD box protein 58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG 1, RIG I, RIG-1/DDX58, RIG-I

验证数据展示

  • IF Staining of HepG2 using CL488-67556

    IF Staining of HepG2 using CL488-67556

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated RIG-1/DDX58 antibody (CL488-67556, Clone: 3B10F5 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67556-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67556 targets RIG-1/DDX58 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen RIG-1/DDX58 fusion protein Ag18591 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Synonyms DDX58, DEAD box protein 58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG 1, RIG I, RIG-1/DDX58, RIG-I
Calculated Molecular Weight 925 aa, 106 kDa
Observed Molecular Weight 101/106 kDa
GenBank Accession NumberBC132786
Gene Symbol RIG-1/DDX58
Gene ID (NCBI) 23586
RRIDAB_2919499
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95786
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58,which has two isoform with the MW of 106 and 101 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RIG-1/DDX58 antibody CL488-67556Download protocol
Standard Protocols
Click here to view our Standard Protocols