验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, RAW 264.7 cells, HSC-T6 cells, Ramos cells, K-562 cells |
Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66647-1-Ig targets RNF126 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, FC (Intra), ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | RNF126 fusion protein Ag13816 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | ring finger protein 126 |
别名 | RING finger protein 126, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF126, 1E1B10 |
计算分子量 | 326 aa, 36 kDa |
观测分子量 | 36-38 kDa |
GenBank蛋白编号 | BC001442 |
基因名称 | RNF126 |
Gene ID (NCBI) | 55658 |
RRID | AB_2858189 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9BV68 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).
实验方案
Product Specific Protocols | |
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WB protocol for RNF126 antibody 66647-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Mol Cell RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.
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Exp Eye Res Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex. | ||
Cell Death Dis ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination. | ||
Cell Chem Biol Bortezomib inhibits ZIKV/DENV by interfering with viral polyprotein cleavage via the ERAD pathway | ||
J Cell Physiol ZNF263 cooperates with ZNF31 to promote the drug resistance and EMT of pancreatic cancer through transactivating RNF126 | ||
ACS Cent Sci DCAF16-Based Covalent Handle for the Rational Design of Monovalent Degraders
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