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RNF126 Monoclonal antibody

RNF126 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66647-1-Ig

产品说明书

CloneNo. 1E1B10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA and More (2)

RING finger protein 126, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF126, 1E1B10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, RAW 264.7 cells, HSC-T6 cells, Ramos cells, K-562 cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66647-1-Ig targets RNF126 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RNF126 fusion protein Ag13816 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ring finger protein 126
别名 RING finger protein 126, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF126, 1E1B10
计算分子量 326 aa, 36 kDa
观测分子量 36-38 kDa
GenBank蛋白编号BC001442
基因名称 RNF126
Gene ID (NCBI) 55658
RRIDAB_2858189
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BV68
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).

实验方案

Product Specific Protocols
WB protocol for RNF126 antibody 66647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.

Authors - Xianyan Hu
  • KD Validated
humanWB

Exp Eye Res

Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.

Authors - Jing Zhang
humanWB

Cell Death Dis

ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.

Authors - Shengjun Geng
humanWB

Cell Chem Biol

Bortezomib inhibits ZIKV/DENV by interfering with viral polyprotein cleavage via the ERAD pathway

Authors - Yali Ci
humanIHC,IF

J Cell Physiol

ZNF263 cooperates with ZNF31 to promote the drug resistance and EMT of pancreatic cancer through transactivating RNF126

Authors - Jiawei Zhang
humanWB

ACS Cent Sci

DCAF16-Based Covalent Handle for the Rational Design of Monovalent Degraders

Authors - Melissa Lim
  • KO Validated
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