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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 66647-1-Ig (same clone as 66647-1-PBS)

    WB analysis of Jurkat using 66647-1-Ig (same clone as 66647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 66647-1-Ig (same clone as 66647-1-PBS)

    FC experiment of Jurkat using 66647-1-Ig (same clone as 66647-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Monoclonal antibody (66647-1-Ig, Clone:1E1B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RNF126 Monoclonal antibody, PBS Only

RNF126 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 66647-1-PBS

Print datasheet

CloneNo. 1E1B10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

RING finger protein 126, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF126, 1E1B10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66647-1-PBS targets RNF126 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RNF126 fusion protein Ag13816 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ring finger protein 126
Synonyms RING finger protein 126, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF126, 1E1B10
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC001442
Gene Symbol RNF126
Gene ID (NCBI) 55658
RRIDAB_2858189
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BV68
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).