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  • Featured Product
  • KD/KO Validated

RRM1 Monoclonal antibody, PBS Only

RRM1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5H6F3

Cat No : 60073-2-PBS

Print datasheet

Synonyms

RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1

验证数据展示

  • WB analysis of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    WB analysis of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RRM1 antibody included
  • IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RRM1 antibody included
  • IF Staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    IF Staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60073-2-Ig (same clone as 60073-2-PBS)

    IF Staining of HepG2 using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    IP experiment of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    IP result of anti-RRM1 (IP:60073-2-Ig, 5ug; Detection:60073-2-Ig 1:1000) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.

产品信息

60073-2-PBS targets RRM1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RRM1 fusion protein Ag0789 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ribonucleotide reductase M1
Synonyms RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23921
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).