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RRM1 Monoclonal antibody

RRM1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

5H6F3

Cat no : 60073-2-Ig

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Synonyms

RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1



经过测试的应用

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human urothelial carcinoma tissue, human colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:3000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60073-2-Ig targets RRM1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen RRM1 fusion protein Ag0789 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ribonucleotide reductase M1
Synonyms RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_10597538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23921
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).

实验方案

Product Specific Protocols
WB protocol for RRM1 antibody 60073-2-IgDownload protocol
IHC protocol for RRM1 antibody 60073-2-IgDownload protocol
IF protocol for RRM1 antibody 60073-2-IgDownload protocol
IP protocol for RRM1 antibody 60073-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Mol Med

PMID: 31950591

Authors - Sean G Rudd
humanIF

Head Neck

ATM, THMS and RRM1 protein expression in nasopharyngeal carcinomas (NPC) treated with curative intent.

Authors - Jenny Jaeeun Ko
humanWB

J Gastrointest Oncol

Abrogation of ARF6 in promoting erastin-induced ferroptosis and mitigating capecitabine resistance in gastric cancer cells.

Authors - Donghua Geng
humanIHC

Oncol Lett

PMID: 28521448

Authors - Toru Aoyama
humanIHC

Zhongguo Fei Ai Za Zhi

[Prognostic Analysis of ERCC1, RRM1 and p53 Expressions in Postoperative Stage I-II Lung Cancer.].

Authors - Huimin Wang
humanWB,IF

Cell Death Discov

Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.

Authors - Yang Gao
  • KD Validated