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验证数据展示

  • IF Staining of HepG2 using CL488-60073

    IF Staining of HepG2 using CL488-60073

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RRM1 antibody (CL488-60073, Clone: 5H6F3 ) at dilution of 1:200.

  • IF Staining of human breast cancer using CL488-60073

    IF Staining of human breast cancer using CL488-60073

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 RRM1 antibody (CL488-60073, Clone: 5H6F3 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-60073

    FC experiment of HepG2 using CL488-60073

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RRM1 (CL488-60073, Clone:5H6F3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated RRM1 Monoclonal antibody

RRM1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL488-60073

产品说明书

CloneNo. 5H6F3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, IF-P, FC (Intra)

别名

RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60073 targets RRM1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 human
免疫原 RRM1 fusion protein Ag0789 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ribonucleotide reductase M1
别名 RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1
计算分子量 90 kDa
观测分子量 90 kDa
GenBank蛋白编号BC006498
基因名称 RRM1
Gene ID (NCBI) 6240
RRIDAB_2883095
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23921
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RRM1 antibody CL488-60073Download protocol
FC protocol for CL Plus 488 RRM1 antibody CL488-60073Download protocol
Standard Protocols
Click here to view our Standard Protocols