CoraLite®594-conjugated RRM1 Monoclonal antibody
RRM1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
5H6F3
验证数据展示
经过测试的应用
| Positive IF-P detected in | human breast cancer tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60073 targets RRM1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IF-P, FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | RRM1 fusion protein Ag0789 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ribonucleotide reductase M1 |
| Synonyms | RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1 |
| Calculated Molecular Weight | 90 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC006498 |
| Gene Symbol | RRM1 |
| Gene ID (NCBI) | 6240 |
| RRID | AB_2883415 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P23921 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 RRM1 antibody CL594-60073 | Download protocol |
| FC protocol for CL594 RRM1 antibody CL594-60073 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
