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CoraLite®594-conjugated RRM1 Monoclonal antibody

RRM1 Monoclonal Antibody for FC (Intra), IF/ICC,IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra), IF/ICC,IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5H6F3

Cat No : CL594-60073

Print datasheet

Synonyms

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1

验证数据展示

  • IF Staining of HepG2 using CL594-60073

    IF Staining of HepG2 using CL594-60073

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60073 (RRM1 antibody) at dilution of 1:100.

  • IF Staining of human breast cancer using CL594-60073

    IF Staining of human breast cancer using CL594-60073

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 RRM1 antibody (CL594-60073, Clone: 5H6F3 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-60073

    FC experiment of HepG2 using CL594-60073

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RRM1 (CL594-60073, Clone:5H6F3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells, human breast cancer tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60073 targets RRM1 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra), IF/ICC,IF-P
Tested Reactivity human
Immunogen RRM1 fusion protein Ag0789 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ribonucleotide reductase M1
Synonyms R1, ribonucleotide reductase M1, RIR1, RR1, RRM1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_2883415
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23921
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).

实验方案

Product Specific Protocols
IF protocol for CL594 RRM1 antibody CL594-60073Download protocol
FC protocol for CL594 RRM1 antibody CL594-60073Download protocol
Standard Protocols
Click here to view our Standard Protocols