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验证数据展示

  • WB analysis of A549 using 19686-1-AP

    WB analysis of A549 using 19686-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19686-1-AP

    IF Staining of HepG2 using 19686-1-AP

    Immunofluorescent analysis of HepG2 cells, using RRM1 antibody 19686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

RRM1 Polyclonal antibody

RRM1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19686-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

RRM1-Specific, RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19686-1-AP targets RRM1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribonucleotide reductase M1
别名 RRM1-Specific, RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1
计算分子量 90 kDa
观测分子量 90 kDa
GenBank蛋白编号NM_001033
基因名称 RRM1
Gene ID (NCBI) 6240
RRIDAB_10697654
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23921
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RRM1, also named as RR1, belongs to the ribonucleoside diphosphate reductase large chain family. RRM1 provides the precursors necessary for DNA synthesis. RRM1 catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.RRM1 is a key enzyme in DNA synthesis: low level expression of RRM1 is associated with poor survival. RRM1 is a prognostic marker of NSCLC. The antibody is specfic to RRM1.

实验方案

Product Specific Protocols
WB protocol for RRM1 antibody 19686-1-APDownload protocol
IF protocol for RRM1 antibody 19686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols