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RRM1-Specific Polyclonal antibody

RRM1-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19686-1-AP

Print datasheet

Synonyms

RRM1, RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1

验证数据展示

  • WB analysis of A549 using 19686-1-AP

    WB analysis of A549 using 19686-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19686-1-AP

    IF Staining of HepG2 using 19686-1-AP

    Immunofluorescent analysis of HepG2 cells, using RRM1 antibody 19686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19686-1-AP targets RRM1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonucleotide reductase M1
Synonyms RRM1, RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberNM_001033
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_10697654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23921
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RRM1, also named as RR1, belongs to the ribonucleoside diphosphate reductase large chain family. RRM1 provides the precursors necessary for DNA synthesis. RRM1 catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.RRM1 is a key enzyme in DNA synthesis: low level expression of RRM1 is associated with poor survival. RRM1 is a prognostic marker of NSCLC. The antibody is specfic to RRM1.

实验方案

Product Specific Protocols
WB protocol for RRM1-Specific antibody 19686-1-APDownload protocol
IF protocol for RRM1-Specific antibody 19686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols