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CoraLite® Plus 488-conjugated RanBP1 Polyclonal antibody

RanBP1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27804

Print datasheet

Synonyms

RAN binding protein 1

验证数据展示

  • IF Staining of NIH/3T3 using CL488-27804

    IF Staining of NIH/3T3 using CL488-27804

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 RanBP1 antibody (CL488-27804) at dilution of 1:200, CL594-phalloidin (red).

  • IF Staining of HeLa using CL488-27804

    IF Staining of HeLa using CL488-27804

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RanBP1 antibody (CL488-27804) at dilution of 1:200.

  • FC experiment of HeLa using CL488-27804

    FC experiment of HeLa using CL488-27804

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated RanBP1 Polyclonal antibody (CL488-27804)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RANBP1 Ready-To-Use IHC Kit. RANBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27804-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27804 targets RanBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen RanBP1 fusion protein Ag25714 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAN binding protein 1
Synonyms RAN binding protein 1
Observed Molecular Weight 28 kDa
Gene Symbol RANBP1
Gene ID (NCBI) 5902
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43487
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RanBP1 (Ran-binding protein 1) belongs to the RANBP1 family. RanBP1 plays a role in RAN-dependent nucleocytoplasmic transport. It alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. And RanBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RanBP1 promotes dissociation of RAN from a complex with KPNA2 and CSE1L. It required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PMID:17671426). Also, RanBP1 inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PMID:7882974, PMID:7616957).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RanBP1 antibody CL488-27804Download protocol
FC protocol for CL Plus 488 RanBP1 antibody CL488-27804Download protocol
Standard Protocols
Click here to view our Standard Protocols