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Rat VEGF 164 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: rat

Applications

Sandwich ELISA

Range

Sandwich ELISA: 31.25-2000 pg/mL

Cat No : MP00598-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00598-3, Rat VEGF Recombinant Matched Antibody Pair - PBS only. 83610-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0941. 83610-2-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

    Sandwich ELISA standard curve of MP00598-3

    Sandwich ELISA standard curve of MP00598-3, Rat VEGF Recombinant Matched Antibody Pair - PBS only. 83610-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0941. 83610-2-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Serum of eight rats was measured. The VEGF 164 concentration of one sample was determined to be 34.3 pg/mL, and the range of VEGF 164 concentration was 0 - 34.3 pg/mL.

    Sample test of MP00598-3

    Serum of eight rats was measured. The VEGF 164 concentration of one sample was determined to be 34.3 pg/mL, and the range of VEGF 164 concentration was 0 - 34.3 pg/mL.

  • Rat lung, cut into 1-2 mm pieces, was cultured for 4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The cell culture supernatant was removed, assayed for rat VEGF, and measured 3292.9 pg/mL. Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernatant was removed, assayed for rat VEGF, and measured 433.1 pg/mL.

    Sample test of MP00598-3

    Rat lung, cut into 1-2 mm pieces, was cultured for 4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The cell culture supernatant was removed, assayed for rat VEGF, and measured 3292.9 pg/mL. Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernatant was removed, assayed for rat VEGF, and measured 433.1 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00598-3 targets VEGF 164 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 31.25-2000 pg/mL (Sandwich ELISA);

Capture antibody: Rat VEGF 164 Recombinant antibody, PBS Only (Capture/Detector) 83610-1-PBS (240573B1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Rat VEGF 164 Recombinant antibody, PBS Only (Capture/Detector) 83610-2-PBS (240573B3). 100 μg. Concentration 1 mgl/ml.

Alternative VEGF 164 matched antibody pairs: MP00598-1, MP00598-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83610-1-PBS
Tested Reactivity rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms Vegfa, VEGF164, Vegf, 240573B1
GenBank accession numberNM_001287108.1
Gene symbol Vegfa
UniprotP16612-2
Gene ID (NCBI) 83785
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83610-2-PBS
Tested Reactivity rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms Vegfa, VEGF164, Vegf, 240573B3
GenBank accession numberNM_001287108.1
Gene symbol Vegfa
UniprotP16612-2
Gene ID (NCBI) 83785
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C