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验证数据展示

  • WB analysis of mouse brain using 55485-1-AP

    WB analysis of mouse brain using 55485-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55485-1-AP (Rootletin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 55485-1-AP

    IF Staining of hTERT-RPE1 using 55485-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Rootletin antibody (55485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

Rootletin Polyclonal antibody

Rootletin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 55485-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

CROCC, KIAA0445, ROLT, Rootletin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy CROCC/Rootletin Ready-To-Use IHC Kit. CROCC/Rootletin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55485-1-AP targets Rootletin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ciliary rootlet coiled-coil, rootletin
别名 CROCC, KIAA0445, ROLT, Rootletin
计算分子量 229 kDa
观测分子量228 kDa
GenBank蛋白编号NM_014675
基因名称 Rootletin
Gene ID (NCBI) 9696
RRIDAB_2935543
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TZA2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rootletin is predicted to enable kinesin binding activity and structural molecule activity. Involved in several processes, including centriole-centriole cohesion; positive regulation of cilium assembly; and positive regulation of protein localization to cilium.

实验方案

Product Specific Protocols
WB protocol for Rootletin antibody 55485-1-APDownload protocol
IF protocol for Rootletin antibody 55485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols