验证数据展示
经过测试的应用
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Planning an IHC experiment? We recommend our IHCeasy CROCC/Rootletin Ready-To-Use IHC Kit. CROCC/Rootletin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55485-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-55485 targets Rootletin in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ciliary rootlet coiled-coil, rootletin |
| 别名 | CROCC, KIAA0445, ROLT, Rootletin |
| 计算分子量 | 229 kDa |
| 观测分子量 | 228 kDa |
| GenBank蛋白编号 | NM_014675 |
| 基因名称 | Rootletin |
| Gene ID (NCBI) | 9696 |
| RRID | AB_3672859 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity Purified |
| UNIPROT ID | Q5TZA2 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rootletin is predicted to enable kinesin binding activity and structural molecule activity. Involved in several processes, including centriole-centriole cohesion; positive regulation of cilium assembly; and positive regulation of protein localization to cilium.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Rootletin antibody CL488-55485 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |