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验证数据展示

  • WB analysis using 10237-1-AP

    WB analysis using 10237-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 cells using 10237-1-AP

    WB analysis of PC-3 cells using 10237-1-AP

    WB result of S100A11 antibody (10237-1-AP, 1:4000) with si-control and si-S100A11 transfected PC-3 cells.

  • WB analysis using 10237-1-AP

    WB analysis using 10237-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 10237-1-AP

    IP experiment of PC-3 using 10237-1-AP

    IP result of anti-S100A11 (IP:10237-1-AP, 3ug; Detection:10237-1-AP 1:1000) with PC-3 cells lysate 1000ug.

  • IP experiment of DU 145 using 10237-1-AP

    IP experiment of DU 145 using 10237-1-AP

    IP result of anti-S100A11 (IP:10237-1-AP, 4ug; Detection:10237-1-AP 1:5000) with DU 145 cells lysate 1280 ug.

  • IHC staining of human cervical cancer using 10237-1-AP

    IHC staining of human cervical cancer using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • IHC staining of human lung cancer using 10237-1-AP

    IHC staining of human lung cancer using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • IHC staining of human thyroid cancer using 10237-1-AP

    IHC staining of human thyroid cancer using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • IHC staining of mouse kidney using 10237-1-AP

    IHC staining of mouse kidney using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • IHC staining of rat kidney using 10237-1-AP

    IHC staining of rat kidney using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • IHC staining of human liver cancer using 10237-1-AP

    IHC staining of human liver cancer using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • IHC staining of human breast cancer using 10237-1-AP

    IHC staining of human breast cancer using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10237-1-AP (S100A11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • IHC staining of human breast cancer using 10237-1-AP

    IHC staining of human breast cancer using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10237-1-AP (S100A11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • IF Staining of PC-3 using 10237-1-AP

    IF Staining of PC-3 using 10237-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using S100A11 antibody (10237-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of BxPC-3 using 10237-1-AP

    IF Staining of BxPC-3 using 10237-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using S100A11 antibody (10237-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of PC-3 using 10237-1-AP

    FC experiment of PC-3 using 10237-1-AP

    1x10^6 PC-3 cells were intracellularly stained with 0.8 ug S100A11 Polyclonal antibody (10237-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human colon using 10237-1-AP

    IHC staining of human colon using 10237-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A11 antibody included
  • Featured Product
  • KD/KO验证

S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10237-1-AP

产品说明书

引用文献(51)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

别名

Protein S100-A11, Protein S100 C, Protein S100 A11, MLN70, MLN 70

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, HCT 116 cells, BxPC-3 cells, human lung tissue, HEK-293 cells, human heart tissue, COLO 320 cells, HaCaT cells, U2OS cells, SKOV-3 cells
Positive IP detected inDU 145 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue, human colon tissue, human colon cancer tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human thyroid cancer tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells, BxPC-3 cells
Positive FC (Intra) detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy S100A11 Ready-To-Use IHC Kit. S100A11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10237-1-AP targets S100A11 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, Neutralization, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, Neutralization
经测试反应性 human, rat
文献引用反应性human, mouse, rat
免疫原 S100A11 fusion protein Ag0343 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A11
别名 Protein S100-A11, Protein S100 C, Protein S100 A11, MLN70, MLN 70
计算分子量 105 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC001410
基因名称 S100A11
Gene ID (NCBI) 6282
RRIDAB_2183478
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31949
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

实验方案

Product Specific Protocols
WB protocol for S100A11 antibody 10237-1-APDownload protocol
IHC protocol for S100A11 antibody 10237-1-APDownload protocol
IF protocol for S100A11 antibody 10237-1-APDownload protocol
IP protocol for S100A11 antibody 10237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

S100A11 is required for efficient plasma membrane repair and survival of invasive cancer cells.

Authors - Jyoti K Jaiswal
View Article
humanWB

Dev Cell

Annexin A1 Tethers Membrane Contact Sites that Mediate ER to Endosome Cholesterol Transport.

Authors - Emily R Eden
View Article
humanIHC

J Pathol

Tissue-based proteomics reveals FXYD3, S100A11 and GSTM3 as novel markers for regional lymph node metastasis in colon cancer.

Authors - Meding Stephan S
View Article
humanIF

Mol Cell Proteomics

S100A11 mediates hypoxia-induced mitogenic factor (HIMF)-induced smooth muscle cell migration, vesicular exocytosis, and nuclear activation.

Authors - Fan Chunling C
View Article
humanWB, IF, CoIP

Cell Mol Gastroenterol Hepatol

S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.

Authors - Linqiang Zhang
View Article
human, mouseWB, IHC, IF

J Clin Endocrinol Metab

Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure.

Authors - Liu Xin-Mei XM
View Article