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验证数据展示

  • FC experiment of MCF-7 using CL647-10237

    FC experiment of MCF-7 using CL647-10237

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human S100A11 (CL647-10237) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for FC (Intra)
Cat No. CL647-10237

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

Protein S100-A11, Protein S100 C, Protein S100 A11, MLN70, MLN 70

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy S100A11 Ready-To-Use IHC Kit. S100A11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10237-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10237 targets S100A11 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 S100A11 fusion protein Ag0343 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A11
别名 Protein S100-A11, Protein S100 C, Protein S100 A11, MLN70, MLN 70
计算分子量 105 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC001410
基因名称 S100A11
Gene ID (NCBI) 6282
RRIDAB_2934812
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31949
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 S100A11 antibody CL647-10237Download protocol
Standard Protocols
Click here to view our Standard Protocols