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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-10237

Print datasheet

Synonyms

Protein S100-A11, Protein S100 C, Protein S100 A11, MLN70, MLN 70

验证数据展示

  • IF Staining of PC-3 using CL555-10237

    IF Staining of PC-3 using CL555-10237

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite®555 S100A11 antibody (CL555-10237) at dilution of 1:200.

  • FC experiment of PC-3 using CL555-10237

    FC experiment of PC-3 using CL555-10237

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human S100A11 (CL555-10237) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy S100A11 Ready-To-Use IHC Kit. S100A11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10237-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-10237 targets S100A11 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen S100A11 fusion protein Ag0343 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein A11
Synonyms Protein S100-A11, Protein S100 C, Protein S100 A11, MLN70, MLN 70
Calculated Molecular Weight 105 aa, 12 kDa
GenBank Accession NumberBC001410
Gene Symbol S100A11
Gene ID (NCBI) 6282
RRIDAB_2919583
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31949
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

实验方案

Product Specific Protocols
IF protocol for CL555 S100A11 antibody CL555-10237Download protocol
FC protocol for CL555 S100A11 antibody CL555-10237Download protocol
Standard Protocols
Click here to view our Standard Protocols