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验证数据展示

  • WB analysis of A431 using 82923-1-RR (same clone as 82923-1-PBS)

    WB analysis of A431 using 82923-1-RR (same clone as 82923-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 82923-1-RR (S100A16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82923-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82923-1-RR (same clone as 82923-1-PBS)

    FC experiment of A431 using 82923-1-RR (same clone as 82923-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A16 Recombinant antibody (82923-1-RR, Clone:3E1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82923-1-PBS in a different storage buffer formulation.

S100A16 Recombinant monoclonal antibody, PBS Only

S100A16 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82923-1-PBS

产品说明书

CloneNo. 3E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

3E1, AAG13, Aging-associated gene 13 protein, Protein S100 A16, Protein S100 F

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82923-1-PBS targets S100A16 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2023

Product name: Recombinant human S100A16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-103 aa of BC019099

Sequence: MSDCYTELEKAVIVLVENFYKYVSKYSLVKNKISKSSFREMLQKELNHMLSDTGNRKAADKLIQNLDANHDGRISFDEYWTLIGGITGPIAKLIHEQEQQSSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A16
别名 3E1, AAG13, Aging-associated gene 13 protein, Protein S100 A16, Protein S100 F
计算分子量 103 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC019099
基因名称 S100A16
Gene ID (NCBI) 140576
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96FQ6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).

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