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验证数据展示

  • WB analysis of A431 using 82923-1-RR

    WB analysis of A431 using 82923-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 82923-1-RR (S100A16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 82923-1-RR

    FC experiment of A431 using 82923-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A16 Recombinant antibody (82923-1-RR, Clone:3E1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

S100A16 Recombinant antibody

S100A16 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82923-1-RR

产品说明书

CloneNo. 3E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Protein S100-A16, Protein S100 F, Protein S100 A16, Aging-associated gene 13 protein, AAG13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82923-1-RR targets S100A16 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 S100A16 fusion protein Ag2023 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A16
别名 Protein S100-A16, Protein S100 F, Protein S100 A16, Aging-associated gene 13 protein, AAG13
计算分子量 103 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC019099
基因名称 S100A16
Gene ID (NCBI) 140576
RRIDAB_3670658
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96FQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).

实验方案

Product Specific Protocols
WB protocol for S100A16 antibody 82923-1-RRDownload protocol
FC protocol for S100A16 antibody 82923-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols