验证数据展示
经过测试的应用
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells, HeLa cells |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84516-6-RR targets SAE1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | SAE1 fusion protein Ag0275 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | SUMO1 activating enzyme subunit 1 |
别名 | 241923F12, AOS1, HSPC140, Sentrin/SUMO activating protein AOS1, SUA1 |
计算分子量 | 38 kDa |
GenBank蛋白编号 | BC000344 |
基因名称 | SAE1 |
Gene ID (NCBI) | 10055 |
RRID | AB_3672022 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q9UBE0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for SAE1 antibody 84516-6-RR | Download protocol |
IF protocol for SAE1 antibody 84516-6-RR | Download protocol |
FC protocol for SAE1 antibody 84516-6-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |