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SAE1 Recombinant antibody

SAE1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241923C6

Cat no : 84516-7-RR

Print datasheet

Synonyms

SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140, AOS1

验证数据展示

  • WB analysis using 84516-7-RR

    WB analysis using 84516-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84516-7-RR (SAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84516-7-RR

    FC experiment of HeLa using 84516-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SAE1 Recombinant antibody (84516-7-RR, Clone:241923C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84516-7-RR

    Affinity and Kinetic Characterization of 84516-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84516-7-RR against Human SAE1 were performed. The affinity constant is 1.98 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, A431 cells, HeLa cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84516-7-RR targets SAE1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SAE1 fusion protein Ag0275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SUMO1 activating enzyme subunit 1
Synonyms SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140, AOS1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC000344
Gene Symbol SAE1
Gene ID (NCBI) 10055
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBE0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

实验方案

Product Specific Protocols
WB protocol for SAE1 antibody 84516-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols